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. 2011 Feb 2;6(2):e16691. doi: 10.1371/journal.pone.0016691

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Jaiswal et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Panels A and B represent the protocols of the SN- and LP-BER, respectively. Panel C shows the autoradiogram describing the effect of varying concentrations of NSC-124854 on SN- and LP-BER activities. Lane 1 shows 63-mer ³²P-labeled U-DNA, Lane 2 shows 23-mer product after APE1 incision, Lane 3 shows 1-nt incorporation by Pol-β, Lane 4 shows strand-displacement products after the addition of Fen1. Lanes 5 and 6 show the 63-mer ligated product of SN- and LP-BER activities, respectively. Lane 8-19 shows the effect of NSC-124854 on the blockage of 1-nt (24-mer product) incorporation. Lanes 8-11 depict NSC-124854-mediated block of strand-displacement synthesis. Furthermore, lanes 12–15 and lanes 16–19 show the effect of NSC-124854 on the blockage of SN- and LP-BER activities in a dose-dependent manner. Data is a representative of three different experiments.