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Acute Mcm7 depletion does not trigger a checkpoint response. Quiescent hTERT-expressing HDF were transfected with siMcm7 or siCon oligonucleotides and then stimulated to enter the cell cycle. Some cultures of siCon-transfected cells were treated with 600 nM BPDE to induce DNA damage. 12 hours after serum stimulation cells were lysed and analyzed by SDS-PAGE and immunoblot using the indicated antibodies.
