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. Author manuscript; available in PMC: 2011 Feb 2.
Published in final edited form as: Cell Cycle. 2009 Jan 1;8(1):125–136. doi: 10.4161/cc.8.1.7528

Figure 5.

Figure 5

HPV E7 expression allows S-phase entry of Mcm7-depleted cells. (A) Quiescent HDF were transfected with siCon or siMcm7 for 24 hr. Transfected cells were then infected with AdCon or AdCyclin D1 as indicated. Cells were stimulated with serum for 0 hr, 4 hr and 12 hr. Extracts from the resulting cells were analyzed by SDS-PAGE and immunoblotting with various antibodies as indicated. (B) Quiescent HDF were transfected with siCon or siMcm7, then infected with AdCon or AdE7 as described above. The resulting cells were incubated with 10 μM BrdU for 1 hr, then analyzed by flow cytometry to determine cell cycle distributions. (C) Quiescent HDF were transfected with siCon or siMcm7 for 24 hr. Transfected cells were then infected with AdCon or AdE7 as indicated. After 20 hr of serum stimulation cell extracts were prepared and analyzed by SDS-PAGE and immunoblotting with various as indicated.