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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Nat Methods. 2010 Aug 22;7(9):761–768. doi: 10.1038/nmeth.1493

Figure 2. EB1-EGFP Object Tracking.

Figure 2

(a) Maximum intensity projection of EB1-EGFP time-lapse sequence (75 frames, 0.4 s frame−1). Growing microtubule ends appear as bright tracks. Scale bar, 10 μm. (b) Computer-generated growth tracks (yellow) with a minimum lifetime of 4 frames. (c) Histogram of growth velocities. The red line is the maximum search radius. (d) Histogram of growth track lifetimes. Red line, least-squares fit of a single exponential decay excluding the first data point. (e) Mean growth rates as a function of minimum growth track lifetime. Grey area, standard deviation.