Table 2.

Predicted stability of wild-type and anchor residue-modified pMHCIs using pMHC binding algorithms.

Peptide	aRANKPEP binding score	bSYFPEITHI binding score	cHLA_BIND binding score
KIFGSLAFL	89	26	235
KLFGSLAFV	99	30	11394
SLLMWITQC	62	18	42
SLLMWITQL	89	28	182
VISNDVCAQV	13	21	16
VLSNDVCAQV	16	23	118
YLEPGPVA	−3.7	n/a	n/a
YLEPGPVV	1.5	n/a	n/a

Heteroclitic modifications are shown in bold and underlined.

^aPrediction of peptide binding score to HLA A*0201 using RANKPEP (Reche et al., 2004).

^bPrediction of peptide binding score to HLA A*0201 using SYFPEITHI (Rammensee et al., 1999).

^cPrediction of peptide binding score to HLA A*0201 using HLA_BIND (Parker et al., 1994).