Protein/Liposome Interactions by NMR
(A) Protein/liposome titrations. The ratio RSN (see Experimental Procedures for details) is shown for F2-F3-4E with 20% DMPS/PC liposomes (purple), for F2-4E with DMPS liposomes (orange), for F2-WT with DMPS/PC liposomes (blue), and for F2-4E with DMPS/PC liposomes (red). Values close to 1 imply weak interaction with the liposomes, whereas values near zero imply strong interactions.
(B) The value of RSN is compared for the different proteins at a concentration of 100 μM. The same color code as in the previous figure is used. The ratio RSN is mean value ± standard deviation from the signal/noise ratio with and without the liposomes for every peak in each system. See also Figure S1 and Figure S2.