Fig. 1.

A Giemsa stained, EDTA-anticoagulated, peripheral blood smear (A) was made prior to collection of tissue sections from a M. haemofelis infected cat (HF11) 19 DPI when approximately 70% of RBCs had one or more organisms attached. Formalin-fixed, paraffin wax-embedded tissue sections were examined by FISH, using a DIG-labelled 16S rDNA probe, tyramide amplification and DTAF-labelled Streptavidin, with DAPI counterstain. M. haemofelis organisms (green) appear on the surface of RBCs within the glomeruli of the kidney (B+), sinusoids of the liver (C+), and blood vessels of other organs, including the jejunum (D+). The positive signal from M. haemofelis was not seen when a 100-fold excess of unlabelled probe was added to the hybridisation buffer (B−, C− and D−). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article).