Figure 3.

Examples of PS-regulated splicing events. Alternative splicing events in the sesB/Ant2 (A), bmm (B), and dre4 (C) genes were identified from the RNA-seq data and validated by RT-PCR. In each case, the RNA-seq coverage and splicing patterns for both the untreated (black) and ps(RNAi) (red) samples are shown along with the annotated transcript models. The RT-PCR validation assays were performed in biological duplicates for both the untreated (lanes 1,2) and ps(RNAi) samples (lanes 3,4). The number of read counts supporting each splicing event in each sample is indicated in the tables on the right.