Figure 5.

Global characteristics of Z2/Z3-enriched gene expression. (A) Z2/Z3-expressed genes are underrepresented on the X chromosome. The chromosomal locations of genes expressed in the adult germline (“all germline”) (Reinke et al. 2004), genes identified from tiling array profiles of Z2/Z3 minus hypodermis-expressed genes (“Z2/Z3 core”, see text), and genes identified from tiling array profiles of adult hermaphrodite somatic cells (“soma-only”) were determined. Observed/expected was calculated by comparing the number of genes per chromosome from each data set to the entire number of protein-coding genes on that chromosome. (B) Meiotic proteins are expressed in Z2/Z3. Antibodies to HTP-3 and REC-8 were used to stain mixed stage embryos, along with PGL-1 or PGL-3 to mark P granules in Z2/Z3. Stage of germline development is indicated on the left, with the earliest stage shown (P2/P3) likely showing persistence of maternal protein, the P4 stage showing decreased protein in the P4 cell, and the Z2/Z3 stage showing an increase in protein level in Z2 and Z3.