Table 2.
Thermodynamic parameters for SRC1 peptide binding to the indicated CAR:RXR complexesa
| Complex | Kd (μM) | ΔH (kcal/mol) | −TΔS (kcal/mol) | ΔG (kcal/mol) | n |
|---|---|---|---|---|---|
| L343A (no ligand) | 14 ± 1.7 | −10.6 ± 0.5 | 4.0 | −6.6 | 1.0 ± 0.13 |
| L343A (TCPOBOP) | 2.0 ± 0.24 | −11.5 ± 0.8 | 3.7 | −7.8 | 0.92 ± 0.14 |
| L343F (no ligand) | 0.92 ± 0.05 | −8.5 ± 0.2 | 0.30 | −8.2 | 0.97 ± 0.05 |
| L343F (androstenol) | 4.2 ± 0.29 | −10.0 ± 0.2 | 2.7 | −7.3 | 1.0 ± 0.04 |
a
Determined at 25°C and pH 7.2 as described in materials and methods. The reported values are the average of at least three experiments and the errors are the standard deviation.