Toluidine blue-stained cross-sections of fresh prostatectomy specimens. Typical images of toluidine blue-stained prostate cross-section (n = 10 cross-sections). All photomicrographs are from the same cross-section prepared as described in Materials and Methods under Sample acquisition. An area of benign-appearing glands is depicted in A–C. An area of apparent cancerous-appearing glands is depicted in D–F. The arrows indicate the area used for further magnification. A and D, magnification, ×10; B and E, magnification, ×30; C and F, magnification, ×100. Compare gland size, closeness, and regularity between B and E or between C and F.