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NUMB is expressed in gonadotropes in the anterior lobe. NUMB (red) is expressed in many but not all LH (LHβ, panel A) and FSH (FSHβ, panel B) cells. NUMB is not expressed in PRL- (C), ACTH- (POMC, D), GH- (E), or TSH- (TSHβ, F) producing cells. All hormones stained in green. Open arrowheads identify colocalizing cells, arrows show hormone-positive, NUMB-negative cells, whereas closed arrowheads show NUMB-positive, hormone-negative cells. Pictures taken at ×400. Scale bar, 50 μm.
