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. 2010 Nov 17;152(1):69–81. doi: 10.1210/en.2010-0498

Figure 1.

Figure 1

A and B, Genotyping of representative organs from adult (A) or 21-d (B) male deletion mutant or littermate control mice. Gel A in both figures shows the presence of the Cre-recombinase transgene in the mutant mice as a band at 166 bp. TCR-δ (TCR-Δ) is the T cell receptor-δ housekeeping gene (200 bp). Littermate controls and the wt animal have only TCR-Δ. Also included is DNA from a Cre+ animal. Gel B in both figures shows floxed exon 17 of the leptin receptor gene in the pituitaries of these mice. Both alleles are floxed, so the product appears as a 249-bp band in all control and mutant mice. A wt control mouse shows only the 180-bp wild-type Lepr. Gel B also shows the presence of truncated Lepr (with exon 17 deleted) as a 228-bp band (LeprΔΔ), only in the pituitaries of the adult and 21-d Cre+ males (arrow). Gels C and D show genotyping of hypothalami and testes from these same mice, which have only the 249-bp floxed Lepr exon 17 band.