Figure 5.

Reduction in LEPRb selectively in male mice somatotropes. A, The expression of LEPRb by somatotropes is reduced significantly in the mutant (asterisk) along with a significant reduction overall in cells with GH antigens (P < 0.001, star). B and C, Illustration of dual-labeled somatotropes from control fields showing the label for LEPRb as black patches (arrows) in GH cells (double asterisks). GH cell in C (single asterisk) shows only one patch of label for LEPRb in that focal plane. D and E, Illustration of GH cells from deletion mutant mice (# sign), The GH cells show no black patches of label for LEPRb. The pale orange label for GH can still be seen. Cell labeled with @ is labeled for LEPRb but not GH. u, Unlabeled cells. Bar, 10 μm.