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. 2010 Nov 17;152(1):193–206. doi: 10.1210/en.2010-0802

Figure 5.

Figure 5

Effect of T on the myogenic differentiation of hSKMCs in vitro. A, hSKMCs were shifted at high confluence in DM with and without T, supplemented daily for 3 d, and immunostained for MyHC. Myotube area was calculated as in Fig. 4. B, Western blotting of protein extracted from samples as in A; proteins were run in two separate gels; molecular mass expressed in kDa. C, Densitometry of films developed in B. T supplementation increases myotube area and the expression level of MyHC and myogenin. D and E, hSKMCs were grown in GM with and without 100 nm T, supplemented every 2 d for 4 d, then shifted to DM with and without 400 nm T, supplemented daily for 3 d. Myotube area (D) and fusion index (E) were calculated as in Fig. 4. T supplementation in both GM and DM increased myotube area and formed bigger myotubes. Results in A, D, and E are means ± sem of three independent experiments. **, P < 0.01; ***, P < 0.001. Results in B and C represent one of three independent experiments.