Table 1. Summary of promoter/attenuator mutations and porin changes in 18 clinical strains of Escherichia coli, arranged by pulsed-field typea.
| PFGE type | Strain | MIC (μg/mL) |
Patient Location | Promoter mutations | Attenuator mutations | Outer membrane protein profile | |||
|---|---|---|---|---|---|---|---|---|---|
| FOX | CAZ | CRO | |||||||
| A |
QE1 |
16 |
<8 |
<8 |
HCC |
T insertion (–13) to consensus spacer (17 bp) |
G to A, right stem; C to T, upstream of stem/loop |
No abnormalities |
|
| A1 |
QE2 |
8 |
<8 |
<8 |
FD |
T insertion (–13) to consensus spacer (17 bp) |
C to T, upstream of stem/loop |
No abnormalities |
|
| A2 |
QE3 |
>32 |
<8 |
<8 |
Inpatient Ward A |
T insertion (–13) to consensus spacer (17 bp) |
C to T, upstream of stem/loop; C deletion, right stem |
No abnormalities |
|
| A2 |
QE4 |
16 |
<8 |
<8 |
FD |
T insertion (–13) to consensus spacer (17 bp) |
C to T, upstream of stem/loop |
No abnormalities |
|
| A3 |
QE5 |
16 |
<8 |
<8 |
ER |
T insertion (–13) to consensus spacer (17 bp) |
C to T, upstream of stem/loop; C to T, left stem |
No abnormalities |
|
| A4 |
QE6 |
>32 |
<8 |
<8 |
HCC |
T insertion (–13) to consensus spacer (17 bp) |
C to T, upstream of stem/loop |
No abnormalities |
|
| A5 |
QE7 |
>32 |
<8 |
<8 |
HCC |
T insertion (–13) to consensus spacer (17 bp) |
C to T, upstream of stem/loop; ATG deletion in loop/right stem (+27 –29) |
No abnormalities |
|
| A |
QE8 |
16 |
<8 |
<8 |
FD |
T insertion (–13) to consensus spacer (17 bp) |
C to T, upstream of stem/loop; G to A, left stem |
No abnormalities |
|
| A |
QE9 |
>32 |
16 |
<8 |
Inpatient Ward B |
T insertion (–13) to consensus spacer (17 bp) |
C to T, upstream of stem/loop; G to A, right stem |
No abnormalities |
|
| B |
QE10 |
>32 |
<8 |
<8 |
Inpatient Ward C |
C to T (–42); G to A (–18); C to T (–1) |
C to A, left stem; C to T, downstream of stem/loop |
No abnormalities |
|
| C |
QE11 |
>32 |
<8 |
<8 |
Inpatient Ward C |
G to A (–18); C to T (–1) |
C to T, downstream of stem/loop |
No abnormalities |
|
| D |
QE12 |
>32 |
<8 |
<8 |
FD |
G to A (–18); C to T (–1) |
C to T, downstream stem/loop |
Omp F- |
|
| E |
QE13 |
>32 |
16 |
<8 |
FD |
G to A in spacer (–28) |
C to T, downstream stem/loop |
No abnormalities |
|
| F |
QE14 |
8 |
<8 |
<8 |
ER |
T to A (–32), new –35 box; C to T (–11), new –10 box |
None |
No abnormalities |
|
| G |
QE15 |
>32 |
16 |
<8 |
Inpatient Ward D |
T to A (-32), new –35 box |
None |
Omp C- |
|
| H |
QE16 |
>32 |
<8 |
<8 |
HCC |
None |
C to T, downstream stem/loop |
No abnormalities |
|
| I |
QE17 |
16 |
<8 |
<8 |
FD |
None |
C to T, downstream stem/loop |
No abnormalities |
|
| J | QE18 | >32 | <8 | <8 | Inpatient Ward E | None | C deletion, right stem (+31); C to T, downstream stem/loop | No abnormalities | |
aPFGE, pulsed-field gel electrophoresis; FOX, cefoxitin; CAZ, ceftazidime; CRO, ceftriaxone, HCC, Hants Community Clinic; FD, family doctor’s office; ER, emergency room.