Skip to main content
. 2010 Dec 13;20(5):948–961. doi: 10.1093/hmg/ddq541

Figure 6.

Figure 6.

In vitro murine osteoclast assays. (A) Increased Ank mRNA in mature Ank+/+ and AnkKI/KI BMM cultures by qPCR. (B) Rhodamine-phalloidin staining of actin and DAPI nuclear staining of mature osteoclasts. Note the disrupted actin belt (*) in AnkKI/KI BMMs, which are generally smaller in area. (C) Decreased fusion efficiency (percentage of nuclei number in mature osteoclasts to total number of nuclei in culture) of AnkKI/KI BMMs. bP < 0.01. (D) qPCR of DC-Stamp expression in BMMs during osteoclastogenesis. Decreased DC-Stamp mRNA level in day 2 AnkKI/KI BMM cultures containing pre-fusion osteoclasts. bP < 0.01. (E) Cocultures of primary mCOBs and BMMs. Resorption assay evaluated after 12 days of plating on calcium-phosphate coated slides. Replacement with Ank+/+ mCOBs or Ank+/+ BMMs partially rescues resorption activity of AnkKI/KI mCOB or AnkKI/KI BMM cultures, respectively (one-way ANOVA with Tukey's multiple-comparison to the Ank+/+ mCOB-Ank+/+ BMM group, aP < 0.05; bP < 0.01). There is no significant difference between replacement with AnkKI/KI mCOB or AnkKI/KI BMM cultures.