Figure 5.

hAdiPS cells demonstrate pluripotency. (A–C) hAdiPS cells differentiated in culture for 14 days exhibit subpopulations of cells immunoreactive for AFP, SMA and NF. (D–F) Similar immunoreactivity is observed when cryosections of EBs from hAdiPS cells are analyzed (scale bars, 100 µm). (G–I) Analysis of teratomas derived from hAdiPS cells injected in SCID mice shows differentiation of histologically distinct tissue types such as glandular tissue (endoderm), muscle tissue (mesoderm) and neuroepithelial tissue (ectoderm), respectively (scale bars, 100 µm). (J–L) Positive immunostaining for AFP, SMA and NF in teratomas derived from hAdiPS cells injected into SCID mice (scale bars, 50 µm). EBs, embryoid bodies; AFP, alpha-fetoprotein; SMA, smooth muscle actin; NF, neurofilament; SCID, severe combined immunodeficient mice.