Figure 5.

Localization of TOPORS during cell cycle. (A) RPE-1 cells were transfected with plasmid encoding for TOPORS-GFP and GFP alone, fixed and stained for the centrosomal marker γ-tubulin. (B and C) Synchronized RPE-1 cells were fixed and stained for endogenous TOPORS and γ-tubulin. G0 was stained using anti-acetylated α-tubulin antibody. DNA was stained with DAPI. Insets show higher magnifications of centrosome-containing regions. Representative images of each step of the cell cycle are shown. In colored images, TOPORS staining is in green, centrosomes in red, nuclei in blue and the arrow shows the mid-body. Scale bar: 10 μm.