Fig. 3.

Deletion of HDACs Hos3 and Hos2 alters cotranscriptional recruitment of Msl1/Lea1. (A) Graphs depicting the occupancy of Lea1-HA and Msl1-HA at each region of DBP2 in the presence or absence of the HDACs, HOS3 and HOS2, relative to the nontranscribed control. Light gray bars depict the occupancy of Lea1 or Msl1 in the presence of HDACs. Dark gray bars represent Lea1-HA or Msl1-HA occupancy in the absence of HDACs. (B) Graph depicting the occupancy of Lea1-HA or Msl1-HA at each region of ECM33 in the presence and absence of HOS3 and HOS2 relative to the nontranscribed region. Data are represented as in A. Graphs represent the average of three independent experiments, ±SD. (C) Dilution series of the triple deletion mutants, lea1Δ hos3Δ hos2Δ and msl1Δ hos3Δ hos2Δ. Cells were grown at 30 °C in YPD liquid medium until the desired OD₆₀₀ was obtained. Cells were spotted onto YPD plates as a 10-fold serial dilution, and plates were incubated for 2 d at 30 °C, 3 d at 25 °C, and 5 d at 16 °C.