Fig. 1.

Lipidomics of an EMT. (A) (Lower) EMT was introduced in MDCK B cells transformed with an estrogen-inducible and constitutively active Raf1 mutant and cultured in the presence of estrogen and TGF-β. (Upper) Uninduced cells serve as a control. After 4 d, cells were stained for E-cadherin, actin, and nuclei (DAPI). (B) Lipid class compositions of cells that have undergone an EMT compared with control cells. The content of individual lipid classes was determined by summing up absolute abundances of all identified species and is expressed as mol%. An unpaired t test with Welch's correction was used to estimate the P values: *P < 0.05; **P < 0.01. Error bars correspond to SD (n = 3).