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Time course of polarization. MDCK cells were rendered contact-naive by culturing at low density for 3 d, seeded at low density on filters, and allowed to grow for the indicated time periods. (A) Cells were immunostained for podocalyxin (green), β-catenin (red), and nuclei (blue, DAPI). The x-z views of confocal stacks are shown. (B) Immunoblotting analysis of the respective samples for annexin 13b. Equal loading was confirmed by reprobing for β-actin.
