Fig. 1.
DNA-PKcs /ATM double-deficient ES cells are defective in RS joining during extrachromosomal V(D)J recombination. (A) Summary of relative efficiency in SE joining measured by extrachromosomal V(D)J recombination assays in WT, DNA-PKcsΔneo/Δneo, DNA-PKcsΔneo/ΔneoATM−/−, ATM−/−, and XRCC4−/− ES cells. The data presented are the average and SD of at least three independent experiments per cell type per condition. The detailed results of each experiment are presented in Fig. S2. P < 0.01 in all four pairs marked by the brackets (Student t test). (B) Fidelity analysis of the extrachromosomal signal joints recovered from DNA-PKcsΔneo/Δneo, DNA-PKcsΔneo/ΔneoATM−/−, and DNA-PKcsΔneo/Δneo cells treated with ATM inhibitor. (C) Scatter plot for the number of base pairs deleted at signal joints recovered from DNA-PKcsΔneo/Δneo, DNA-PKcsΔneo/ΔneoATM−/−, and DNA-PKcsΔneo/Δneo cells treated with ATM inhibitor. The black bar indicates the mean of each group. The exact sequence of each junction summarized in B and C is presented in Fig. S3.