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. 2011 Jan 18;108(5):2160–2165. doi: 10.1073/pnas.1012232108

Fig. 2.

Fig. 2.

Interactions between SCL3, RGA, and SPY. (A) rga and spy are epistatic to scl3 in the root-length assays in response to 1 μM PAC (10-d-old seedlings). (B) rga is partially epistatic to scl3 in the ga1-3 background (65-d-old plants) (Fig. S2B). (C) In vitro pull-down of RGA with recombinant GST-SCL3. Protein extracts from sly1-10 and sly1-10 rga-24 double mutant were incubated with GST or GST-SCL3 from Escherichia coli separately. − GA, in the absence of GA; + GA, in the presence of 100 μM GA4. Input and pull-down samples were analyzed by immunoblotting using affinity-purified RGA antibody. (D) co-IP of SCL3 and RGA in planta. HA-RGA was transiently expressed alone (RGA only) or coexpressed with cMyc-SCL3 (SCL3/RGA) or cMyc-GUS (GUS/RGA) in N. benthamiana. The nuclear protein extracts were immunoprecipitated with cMyc antibody-conjugated agarose beads, and the input and IP samples were analyzed by immunoblotting using antibodies for cMyc and HA, separately.