Fig. 2.
Watson–Crick conformation of a dGMPCPP•T mispair at the active site. (A) Superposition of the ternary complexes of Pol λ DL (green) in complex with DNA (primer strand is olive, template strand is orange) and incoming dGMPCPP (magenta) opposite template T (orange) and the WT Pol λ ternary complex (2PFO) (protein is gray, template strand is light orange, incoming dUMPNPP is light purple, and template adenine is brown. The metal ions A and B (Me) in the complex of Pol λ DL and WT Pol λ are green and purple, respectively. (B) Close up of the active site of the ternary complex of Pol λ DL wih the dGMPCPP•T nascent mispair. The magnesium ions are green, and active site H2O molecules are light red.