Table 2.

pH dependence of misinsertion

dNTP	Km (μM)	kcat (1/s)	kcat/Km	fmis*
WT Pol λ
pH 7.0
dATP	0.30 ± 0.08	0.02 ± 0.008	6.7 × 10-2 ± 0.9 × 10-2
dGTP	5.1 ± 2.5	0.0003 ± 6.7 × 10-5	5.4 × 10-5 ± 1.5 × 10-5	0.8 × 10-3
pH 9.0
dATP	1.0 ± 0.40	0.012 ± 0.0035	1.2 × 10-2 ± 0.25 × 10-2
dGTP	1.2 ± 0.40	0.001 ± 0.0003	8.6 × 10-4 ± 3.1 × 10-4	72 × 10-3
Pol λ DL
pH 7.0
dATP	0.3 ± 0.06	0.0055 ± 0.0013	1.9 × 10-2 ± 1.5 × 10-2
zdGTP	13 ± 4.2	0.005 ± 0.0035	3.6 × 10-4 ± 1.2 × 10-4	1.9 × 10-2
pH 9.0
dATP	1.4 ± 0.40	0.013 ± 0.0035	1 × 10-2 ± 0.058 × 10-2
dGTP	4.8 ± 1.09	0.012 ± 0.0042	2.5 × 10-3 ± 1.1 × 10-3	25 × 10-2

The kinetic constants (shown with standard deviations) are an average of 3 to 5 independent determinations.

^*1f_mis is the relative efficiency of misincorporation expressed as the ratio of [k_cat/K_mdGTP]/[k_cat/K_mdATP].