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. 2011 Jan 18;108(5):2166–2171. doi: 10.1073/pnas.1012215108

Fig. 2.

Fig. 2.

Regulation of GA-mediated root cell elongation by SCL3–DELLA interaction in the EDZ. The pSCR::gai-GR-YFP seedlings in Col-0, scl3, and 35S::SCL3 were grown in MS agar plates for 4 d, transferred to MS agar plates supplemented with or without 10 μM of DEX, and incubated for another 3 d (A and B) or for 12 h (C–H). In the absence of DEX (−DEX), neither inhibition of root growth nor cell elongation was observed (A, C, E, and G). In the presence of 10 μM of DEX (+DEX) (B, D, F, and H), pSCR::gai-GR-YFP seedlings in Col-0 showed inhibition of root growth and cell elongation. Root growth and cell elongation of pSCR::gai-GR-YFP seedlings in scl3 were more severely inhibited. Notably, overexpression of SCL3 (pSCR::gai-GR-YFP in 35::SCL3) suppressed the inhibition of root growth and cell elongation. As a result, the average cell length is reduced significantly in scl3, whereas increased in 35S::SCL3 (n > 20) (H). (Scale bars, 5 mm in A and B; 30 μm in C and D; and 10 μm in E and F, respectively.) Statistical significance of differences was determined by Student's t test (error bars: SE, **P < 0.01).