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. 2011 Jan 18;108(5):1943–1948. doi: 10.1073/pnas.1010396108

Fig. 3.

Fig. 3.

Inhibition of RhoA-ROCK-Myosin II and β1 integrin impairs uropod formation and invasion in Matrigel. (A–C) MDA-MB-231 cells were treated with the indicated siRNAs targeting RhoA (A), the p34-Arc (ARPC2) subunit of the Arp2/3 actin nucleating complex (B), or β1 integrin (C). Cell lysates were prepared after 72 h, and immunoblotting analysis was performed with indicated antibodies. Of note, knockdown of p34-Arc leads to decreased expression of p16-Arc (B). (D) Quantification of cell invasion by mCh-Lifeact MDA-MB-231 cells plated atop of a thick layer of Matrigel and treated with indicated siRNAs or drugs. Cell invasion was determined by analyzing the proportion of cells buried in Matrigel after 14 h from low magnification scanning electron micrographs. (E) Average migration speed (filled bars) and percentage of cells with uropod (open bars) in MDA-MB-231 cells seeded in Matrigel and treated with indicated siRNA and drugs. Asterisks indicate statistically significant differences compared with control cell populations (Tables S1–S3).