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. 2011 Jan 24;108(5):1868–1873. doi: 10.1073/pnas.1018965108

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Fig. 3. — Interaction between PA pore and LF_N residues 2 and 5 is dependent upon functional Φ-clamp. Shown are EPR spectra of LF_N G2R1 or G5R1 with and without PA F427H, F427S, or F427W collected at pH 5.5 (when PA is in the pore conformation). Black line—LF_N alone. Red line—with PA (1∶1 molar ratio heptamer). Spectra were collected at room temperature and normalized to the intensity of the central peak.

Inline graphic — Interaction between PA pore and LF_N residues 2 and 5 is dependent upon functional Φ-clamp. Shown are EPR spectra of LF_N G2R1 or G5R1 with and without PA F427H, F427S, or F427W collected at pH 5.5 (when PA is in the pore conformation). Black line—LF_N alone. Red line—with PA (1∶1 molar ratio heptamer). Spectra were collected at room temperature and normalized to the intensity of the central peak.