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LFN residues 2 and 5 directly interact with Φ-clamp in PA pore. Black—additive EPR spectra of spin-labeled LFN + spin-labeled PA. Red—EPR spectra of 1∶1 molar ratio spin-labeled LFN:spin-labeled PA complex. Spectra were collected at 233 K at either pH 8.5 (when PA is in the prepore state) or pH 5.5 (when PA is in the pore state) and normalized to the same number of spins.
