Fig. 7.
Interaction between LFN globular domain and PA domain 1′ is independent of PA conformation and Φ-clamp functionality. Shown are EPR spectra of LFN Y108R1 with and without WT PA or PA F427H. Black line—LFN Y108R1 alone. Red line—with WT PA or PA F427H (1∶1 molar ratio LFN∶PA prepore). (Top) Spectra collected at pH 8.5 (when PA is in the prepore conformation). (Bottom) Spectra collected at pH 5.5 (when PA is in the pore conformation). Spectra were collected at room temperature and normalized to the intensity of the central peak.