Fig. 3.

CUX1-induced tumorigenicity involves chromosomal instability. (A) Late-passage NMuMG/vector cells, 2C-sorted NMuMG/CUX1 cells, or aneuploid 8C-sorted NMuMG/CUX1 cells were injected s.c. in nude mice. Tumor volumes (mm³) were measured 27 d after injection. (B) Early-passage NMuMG cells expressing p110 CUX1 or not were pulse-treated with blebbistatin and injected s.c. into nude mice. (C) Chromosome number of cells from six mammary gland tumors arising in p110 or p75 CUX1 transgenic mice was determined from metaphase spreads. **P < 0.0003 for both tumor sizes and tumor numbers.