Table 1.
Validated transcriptional targets activated by CUX1
| mRNA expression (fold) |
||||
| Hs578t shCUX1tetON |
||||
| Gene | ChIP IgG vs. input (fold) | NmuMG (CUX1 vs. vector) | + Dox | − Dox |
| SAC | ||||
| AURKB | +3.8 | +2.6 | −1.3 | +7.0 |
| BUB1* | +3.8 | +3.6 | −2.5 | +5.6 |
| BUB3 | +3.5 | +2.4 | −2.4 | +7.5 |
| BUBR1 | +1.7 | +3.6 | −2.2 | +10.8 |
| CENPE* | +10.8 | +2.6 | −1.6 | +6.7 |
| MAD1L1 | +4.7 | +1.8 | −1.5 | +3.9 |
| MAD2L1* | +2.7 | +2.0 | −2.6 | +5.7 |
| TTK (MPS1) | +2.0 | +3.2 | −1.2 | +5.6 |
| NEK2 | +2.5 | +3.8 | −1.4 | +6.5 |
| ROD | +3.6 | +3.9 | −1.5 | +5.9 |
| ZW10 | +3.1 | +2.0 | −1.8 | +6.3 |
| ZWILCH | +2.9 | +5.0 | −2.5 | +4.3 |
| Centromeric histone | ||||
| CENPA* | +3.1 | +1.9 | −1.5 | +5.8 |
| Kinesin | ||||
| KIF2C* | +2.3 | +3.2 | −1.4 | +6.1 |
| KIFC1* | +1.9 | +2.3 | −1.8 | +9.4 |
| Other functions | ||||
| CAMK2D* | +2.9 | +1.4 | −1.9 | +2.0 |
| COG7* | +8.1 | +1.6 | −1.4 | +1.3 |
| MFAP1* | +4.3 | +1.4 | −1.7 | +3.9 |
| NUDCD1* | +3.7 | +2.6 | −1.2 | +3.8 |
| RIN2* | +4.2 | +2.3 | −1.6 | +2.4 |
| SF3B3* | +3.0 | +2.0 | −1.9 | +4.6 |
Validation of p110 CUX1 transcriptional targets relevant to the establishment of bipolar mitoses. CUX1 transcriptional targets were identified by ChIP in Hs578T breast tumor cells. From left: column 1: asterisks (*) indicate genes previously identified in an siRNA screen for bipolar mitosis (18). Column 2: validation and measurement of promoter occupancy from independent ChIP assays using HeLa cells. Columns 3, 4, and 5: fold difference in mRNA expression measured by real-time PCR between NMuMG/CUX1 and control cells (column 3), Hs578t cells bearing a doxycyclin-inducible CUX1-specific shRNA, treated or not for 5 d with doxycycline (column 4), or after endogenous CUX1 expression was permitted after removal of doxycyclin for 3 d (column 5).