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. 2011 Jan 18;108(5):2118–2123. doi: 10.1073/pnas.1012409108

Fig. 5.

Fig. 5.

MDA5 plays a critical role in the activation of IFN-β by viral mRNA. (A) The roles of RIG-I and MDA5 in the activation of the IFN-β promoter by viral mRNA. The 293T cells were transfected with siRNA targeting RIG-I or MDA5 or with NT siRNA. At 48 h after transfection of siRNA, the cells were transfected with vector, L-II mut, or poly(I):poly(C), along with the luciferase reporter plasmids. Luciferase activity was measured at 18–20 h posttransfection. (B) The roles of RIG-I and MDA5 in induction of IFN-β production by viral mRNA. siRNA transfection was performed as described in Fig. 5A in 293T cells, and at 48 h after transfection with siRNA, the cells were transfected with vector, L-II mut, or poly(I):poly(C). After 18–20 h, the amount of IFN-β in the medium was measured by ELISA. Expression levels of RIG-I, MDA5, and β-actin were examined by immunoblot analysis.