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EGFR and DNAPKcs cellular localisation following IR treatment. Stable NIH3T3 cells expressing wtEGFR, NL123, L858R, LNLS123, EGFRvIII, Vector control were serum starved for 24 hours, treated with 4gy Ionising radiation and then fixed with 4%PFA 20 minutes following treatment. Cells were stained with Goat anti-Rabbit Alexa fluor 647 (EGFR), Goat anti-Mouse Alexa fluor 488 (DNAPKcs), and Dapi (nucleus).
