Figure 2.

RhoA activity is required for PDAC invasion. A,B H&E-stained sections of p53^fl and p53^R172H cells on organotypic matrix. C, Quantification of p53^fl and p53^R172H PDAC cell invasion ± Y27632 or cell-permeable C3 in the organotypic matrix at 8, 12 days. D,E H&E-stained sections of p53^fl cells expressing vector or human mutant p53^R175H cultured on organotypic matrix. Quantification of human mutant p53^R175H-driven PDAC invasion in the organotypic matrix at 8 days. Red arrows depict examples of cells assessed at depth for Fig. 3C. Columns, mean; bars, SE. **, P < 0.01