FIG. 4.

Subcellular localization of Dsc1 in newborn epidermis of wild-type (WT) and mutant (MT) mice. (A) Deconvolution microscopy. The sections were stained with antibody gp899 (Dsc1; red) and a monoclonal antibody against desmoplakin (green). Colocalization resulted in yellow fluorescence. Note that the mutant protein coassembles with desmoplakin into desmosomes. Identical results were obtained by staining with gp899 and a monoclonal antibody against Dsg1 and Dsg2 (DG3.10; data not shown). (B) Low-temperature immunoelectron microscopy. Mutant (a, c, e, g) and wild-type (b, d, f, h) samples were incubated with various antibodies. (a, b) gp899 (silver enhancement; see Materials and Methods). (c, d) Higher magnification of desmosomes stained with gp899. (e, f) Costaining with gp899 (5-nm gold particles) and desmoplakin antibodies (15-nm gold particles). (g, h) Costaining with gp899 (5-nm gold particles) and plakoglobin antibodies (15-nm gold particles). Note that the mutant Dsc1 receptor is integrated into desmosomes and that the staining patterns of desmoplakin and plakoglobin are not affected by the mutation. Bars: a and b, 1 μm; c to h, 0.1 μm.