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Western blot analysis of total-tissue lysates from back skin of newborn wild-type (WT) and dsc1−/−ΔE17LoxP mutant (MT) mice. Equal amounts of protein were blotted with antibodies against Dsg1 and Dsg2 (DG3.10), Dsg3, Dsc3 (gp2280; see Materials and Methods), plakoglobin (Pg), PKP1 (PP1), PKP3 (PP3), and β-catenin (β-cat). No significant differences in the expression levels of the analyzed proteins in wild-type and mutant samples were observed.
