FIG. 8.

Effects of the dsc1 mutation on the expression of Dsc2. (A) RPA analysis of epidermal RNA from newborn wild-type (WT) and dsc1^{−/−ΔE17LoxP} mutant (MT) samples. Note the dramatic increase in Dsc2 expression (approximately 38-fold) in the two mutant samples. β-Actin was used as an internal standard to normalize the expression data. (B) In situ hybridization with Dsc2 antisense probes. Note the strong suprabasal expression of Dsc2 in the mutant sample. The signal obtained with wild-type samples was barely above the background. A sense probe did not yield a signal on mutant or wild-type samples (data not shown). The dotted line indicates the position of the basement membrane. (C) Western blot analysis using total skin extracts from newborn mice with antibody gp2295. Note that Dsc2a and Dsc2b are expressed at similar levels in wild-type and homozygous mutant samples.