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. 2004 Jan;24(1):280–293. doi: 10.1128/MCB.24.1.280-293.2004

FIG. 5.

FIG. 5.

ASK1 inhibits glial differentiation in AHPs. (A) AHPs were cultured without bFGF for 2 days and then infected with adenovirus carrying LacZ, ASK1-KM, or ASK1-ΔN. After another 4 days, cells were processed for Western blot analysis. (B) Densitometric analysis of three independent Western blot assays for GFAP is shown. (C) Immunofluorescence staining for GFAP 4 days after infection. (D) Cell counts of GFAP-positive cells stained 4 and 9 days after infection. (E) AHPs were cultured for 10 days after infection and immunoblotted for GFAP. The values represent means + SEM (n = 3). Two asterisks indicate a P value of <0.01, and three asterisks indicate a P value of <0.001 in comparison to the LacZ-infected control. Results were determined by ANOVA and the post hoc Scheffé test. Scale bar, 10 μM.