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. 2004 Jan;24(1):36–45. doi: 10.1128/MCB.24.1.36-45.2004

FIG. 3.

FIG. 3.

cyPGs activate a set of antioxidant and anti-inflammatory genes in mouse peritoneal macrophages. (A) Peritoneal macrophages were treated with dimethyl sulfoxide alone (lane 1); with 15d-PGJ2 at 0.5 μM (lane 2), 1 μM (lane 3), 5 μM (lane 4), or 10 μM (lane 5) for 5 h; or with 100 μM diethylmaleate for 5 h (lane 6), and total RNAs were analyzed by RNA blot analysis with HO-1, PrxI, and A170 cDNAs as probes. A β-actin cDNA probe was used for a loading control. (B) Peritoneal macrophages were treated with dimethyl sulfoxide alone (lane 1), PGA1 (50 μM) (lane 2), PGB2 (50 μM) (lane 3), PGD2 (50 μM) (lane 4), PGE2 (50 μM) (lane 5), PGF1a (50 μM) (lane 6), 15d-PGJ2 (10 μM) (lane 7), thromboxane B2 (50 μM) (lane 8), leukotriene B4 (1 μM) (lane 9), arachidonic acid (50 μM) (lane 10), or diethylmaleate (100 μM) (lane 11). Total RNA fractions were analyzed by RNA blot analysis as for panel A.