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. 2011 Feb 3;7(2):e1001269. doi: 10.1371/journal.ppat.1001269

Figure 6. Late induction of host response.

Figure 6

qRT-PCR measurement of the expression of IFN genes (top panels; left, splice isoform of ifnϕ1 corresponding to the inducible, secreted cytokine; right, ifnϕ3) and of IFN-induced genes (bottom panels; left, viperin/rsad2, right, MXA). Assays performed on RNA extracted from entire larvae, either infected with ∼100 pfu of IHNV and incubated at 24°C for the indicated time, or uninfected controls (five or four samples, displayed before the “0 hpi” mark). Measured values normalized to the mean of the control set. Error bars, SD of triplicate qPCR measures. Significant difference from control set displayed as ** (p<0.0001) or * (p<0.01); unpaired t-test.