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. 2011 Feb 3;7(2):e1001288. doi: 10.1371/journal.pgen.1001288

Figure 4. Expressing TER94 IBMPFD mutant in neurons does not impair ERAD.

Figure 4

(A and Ai) Fluorescent images of GMR>CD3δ-YFP larval eye discs without (A) or with 5 mM DTT treatment (Ai). (B–Ci) Fluorescent images of GMR>CD3δ-YFP larval eye discs co-expressing indicated transgenes. (D–Fii) Fluorescent images of GMR>CD3δ-YFP larval (D-F) and pupal (30%, Di–Fi; 60% Dii–Fii) eye discs co-expressing LacZ (D–Dii), TER94 wt (E–Eii), and TER94A229E (F–Fii). In all panels, anti-Elav antibody (red) labels the nuclei of retinal neurons. Scale bars, 10 µm (D–F), 5 µm (Di–Fii). Genotypes: (A and Ai) UAS-CD3δ-YFP/w; GMR-GAL4/+, (B) UAS-CD3δ-YFP/w; GMR-GAL4/+; UAS-Sip3-RNAiv6870/+, (Bi) UAS-CD3δ-YFP/w; GMR-GAL4/UAS-Q108, (C)UAS-CD3δ-YFP/w; GMR-GAL4/UAS-TER94K2A, (Ci) w,UAS-CD3δ-YFP/w; GMR-GAL4/UAS-TER94E2Q, (D-Dii) UAS-CD3δ-YFP/w; GMR-GAL4/UAS-LacZ, (E-Eii) UAS-CD3δ-YFP/w; GMR-GAL4/+;UAS-TER94wt/+, (F-Fii) UAS-CD3δ-YFP/w;GMR-GAL4/UAS-TER94A229E.