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. 2011 Feb 3;6(2):e16700. doi: 10.1371/journal.pone.0016700

Figure 2. Expanded gamma delta T cells are selectively cytotoxic against Ph+ leukemia cells and not autologous hematopoietic progenitors.

Figure 2

a) Gamma delta T cells are selectively cytotoxic against Ph+ leukemia cell lines K562 and EM-2, but not against normal autologous hematopoietic progenitors, as assessed by a flow cytometric cytotoxicity assay (n = 9 different donors, minimum 6 replicates/donor). Gamma delta T cells were incubated with target cells for 4 hours at an effector:target ratio of 20∶1. Mean % AnnexinV and %lysis ± standard error of target cells is shown. Data were acquired via FACSCalibur and analysed with CellQuest™ software. b) Co-culture of gamma delta T cells with PBMCs does not adversely affect PBMC growth. 200,000 gamma delta T cells were co-incubated with 10,000 autolougous PBMC at 20∶1 effector:target ratio for 0 or 4 hours as indicated. Subsequently, gamma delta T cells were depleted and of the remaining cells, 150,000 per plate were seeded in triplicate for colony-forming assays for normal hematopoietic progenitors, BFU-E and CFU-GM (n = 3 different donors in triplicate).