Figure 3. EM-2eGFPluc cells are fluorescent and bioluminescent.

a) Scheme of the recombinant lentivector constructed for these studies showing the dual promoter system driving eGFP and Luciferase expression in transduced cells. b) The parental cell line, EM-2, and transduced line, EM-2eGFPluc, were subject to flow cytometry. Shown is a representative example. Cells were assessed by flow cytometry prior to each injection into NSG mice (n = 9). c) EM2eGFPluc cell bioluminescence directly correlates with cell number. Cells were harvested and density adjusted in PBS, then subject to serial dilution 1∶2 from well to well. After addition of luciferin, cells were imaged using IVIS® technology and then quantified using Living Image™ software. Luminescence was plotted against cell number and linear regression calculated using Microsoft Excel software (n = 2).