Figure 1. The effect of aristolochic acid on cell growth of NRK-49f cells.
NRK-49f cells were treated with 0, 3, 6, 12, 25 and 50 µM of AA for 48 h. (A) Cell morphology was investigated by phase-contrast microscopy. Magnification 100X. Scale bar, 50 µM. (B) The cell survival rate was determined by MTT assay, and (C) the cell proliferation was examined by BrdU incorporation. Data are presented as mean±S.D. of 9 replicates from three independent experiments.