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. 2004 Jan;24(1):14–24. doi: 10.1128/MCB.24.1.14-24.2004

FIG.4.

FIG.4.

4HT can elevate endogenous SRC-1 and SRC-3 in HeLa cells transiently transfected with ERα and in MCF-7 cells. (A) HeLa cells were transfected with pERE-E1b-CAT and pCR3.1 hERα, incubated 24 h, and then treated with ethanol vehicle (−), E2, or 4HT for 24 h and harvested for Western analysis. (B) 4HT is able to promote elevation of endogenous SRC-3 in MCF-7 but not T-47D or ZR-75-1 ERα-positive breast cancer cell lines. Untransfected MCF-7, T-47D, and ZR-75-1 cell lines were treated with ligands and harvested for Western analysis as described for panel A. (C) 4HT treatment has no effect on SRC-1 or SRC-3 mRNA levels in HeLa cells transfected with ERα. HeLa cells were transfected with pERE-E1b-CAT and pCR3.1 hERα and then treated for 24 h with ligands as described above. Cells were harvested for total RNA, and mRNA for SRC-1 and SRC-3 was quantitated by real-time PCR. (D) In MG132-treated HeLa cells, SRC-1A-LUC protein is not further elevated when cotreated with 4HT. HeLa cells were transfected with pERE-E1b-CAT, pCR3.1hERα, or pCR3.1 SRC-1A-LUC and treated with ethanol vehicle (−), E2, or 4HT with or without MG132 for 8 h and harvested for luciferase activity.