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. 2011 Feb 3;6(2):e16816. doi: 10.1371/journal.pone.0016816

Figure 3. Neurospheres derived from muscle FDB cultures.

Figure 3

A. Neurospheres formed on day 8 in culture are nestin-GFP+ and Tuj1+. Nuclei were stained with Hoechst 33342. B. Nestin-GFP+ cells grow as monolayers after fusing into neurospheres. C. Nestin-GFP+ cells form networks. D. Nestin-GFP+/Tuj1+ cells exhibit neural morphology, with many processes. Right panels for B, C, and D are brightfield images. Images in B-D were taken on culture day 8. Scale bar  = 100 µm for all pictures, including insets in panel A.