Figure 1. Hedgehog signaling is downregulated during differentiation of hMADS cells.

hMADS cells were kept undifferentiated or differentiated into adipocytes or osteoblasts (A), or treated with increasing concentrations of cyclopamine for 7 days (B). Gli-1 expression was evaluated by real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR). Results are representative of three independent experiments.