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(A): Flow cytometry analysis of hMADS cells treated with cyclopamine for 24H or 5 days and labelled with annexin V and propidium iodide (PI). The percentage of cells positively labelled with annexin V (apoptotic cells) is indicated for each condition. Staurosporine was used as a positive control (0.1 µM for 24H). (B): Percentage of Annexin V-positive cells after a treatment of 5 days with 5 µM cyclopamine.
